Accelerated of Sex Reversal use 17α-methyltestosterone Induced Female, Orange-Spotted Grouper <I>Epinephelus coioides</I>

Highlight Research Sex reversal for orange-spotted grouper Epinephelus coioides The application of 17α-methyltestosterone induce sex change stability need more investigation Abstract occurance hermaphrodites in fish causes a scarcity male parents, so an alternative is needed to accelerate sexchange at young age. present study was expected scrutinize the mechanisms sex-change early process, and whether testis converted from immature ovary using (MT) would recover after termination MT treatment. MT-induced 5-aza-2’-deoxycytidine (5-Aza) were connected as DNA methylation inhibitors comprehend alternation gonadal soma cells. groupers used developmental ages fed diet containing 50 mg/kg three months then normal month. In first week second injected with 5-Aza intraperitoneally during MT-oral administration. Most fishes control group had ovaries, but all females MT, spermatogenesis. However, one month withdrawal treatment, returned female-like even though have undergone masculinization. This outcome demonstrates precocious under yearling, utilizing oral treatment impermanent. All 5-aza treatments showed no spermatogenic this study, lower growth rates demonstrated by MT-treated groups. impact metabolic clear end hormone administration since decreased groups treated months.